PEGylation of a TLR2-agonist-based vaccine delivery system improves antigen trafficking and the magnitude of ensuing antibody and CD8 T cell responses

T Sekiya; J Yamagishi; JHV Gray; PG Whitney; A Martinelli; W Zeng; CY Wong; C Sugimoto; DC Jackson; BY Chua

Journal article

PEGylation of a TLR2-agonist-based vaccine delivery system improves antigen trafficking and the magnitude of ensuing antibody and CD8 T cell responses

T Sekiya, J Yamagishi, JHV Gray, PG Whitney, A Martinelli, W Zeng, CY Wong, C Sugimoto, DC Jackson, BY Chua

Biomaterials | ELSEVIER SCI LTD | Published : 2017

DOI: 10.1016/j.biomaterials.2017.05.018

Abstract

The lipopeptide R4Pam2Cys is an agonist for toll-like receptor-2 (TLR2), a key pathogen-associated molecular pattern receptor expressed on many antigen-presenting cells such as dendritic cells (DCs). Electrostatic association of R4Pam2Cys with soluble protein antigens significantly enhances their immunogenicity and there is evidence to suggest that reducing the size of suitably adjuvanted-antigen complexes in solution may further improve their immunostimulatory capabilities. In this study, we investigated how incorporation of polyethylene glycol (PEG) into R4Pam2Cys affects the size, activity and efficacy of formed antigen-lipopeptide complexes. The presence of PEG was shown to increase solu..

View full abstract

University of Melbourne Researchers

Toshiki Sekiya Author

Weiguang Zeng Author

Chinn Yi Wong Author

David Jackson Author

Related Projects (2)

LIMITING THE IMPACT OF INFLUENZA

The development of better ways to prevent and treat influenza infection will be a major step forward in lessening the impact of the virus in..

THE TLR2 AGONIST PAM2CYS AS AN ANTIMICROBIAL AGENT

Lower respiratory infections are the leading infectious cause of death worldwide. We have discovered a compound that reduces the impact and ..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by grants from the National Health and Medical Research Council of Australia (1071916 and 1063209) and by the Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, founded by the Ministry of Education, Culture, Sports, Science and Technology MEXT, Japan.